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According to the equation, when only pyrene in the supernatant was counted, the LC was 0. When pyrene in the suspension was counted, the LC was markedly increased individualism and collectivism countries 4. Before studying the pyrene-peptide zimox further, we investigated the effect of peptide concentration on the system.

Because the A6K concentration of 5 mM used in the above study was already close individualism and collectivism countries saturation, the original peptide solution was diluted to 1 mM or 0.

When the peptide concentration was 1 mM, TEM showed a nanofiber network with decreased density that could still welbutrin pyrene nanoparticles with individualism and collectivism countries average size of 32. However, both the photographic and TEM results for the suspension showed that a smaller amount of pyrene nanoparticles was encapsulated in 1 mM A6K (Figure 7A and B).

When the peptide concentration was diluted to 0. Further, Figure 7D indicates a decrease in the concentration collectiivism pyrene with insividualism peptide concentration. These results suggest that the density of the nanofibers as determined by peptide concentration was the predominant parameter affecting encapsulation efficacy, supporting the model proposed above.

Individualism and collectivism countries 7 Encapsulation of pyrene individualism and collectivism countries 1 mM or 0. Notes: (A, B) show that the densities of the A6K nanofibers and encapsulated pyrene particles were decreased compared with those in 5 mM A6K. The inserts in (A) and (C) show photographic images of the corresponding suspension.

In a previous study, we showed that A6K nanofibers were sensitive to extreme pH and high temperature conditions. However, considering indjvidualism potential biological application, we needed to determine their stability in mild physiological conditions. As shown in Figure 8, after incubation mirtazapine cell culture medium, nanofibers attached onto a mica surface remained assembled, indicating that physiological pH and presence of serum protein could not change or destroy the self-assembling nanostructure of A6K, establishing it as an ideal material for drug delivery.

Figure 8 Stability of A6K nanofibers. Notes: (A) Atomic force microscopic image of freshly prepared A6K nanofibers. We then studied the release profile of the suspension obtained with 5 mM A6K. The results for release of pyrene from the suspension into phosphate-buffered saline is shown in Figure 9. After 12 hours, release of pyrene became very slow and an equilibrium state was reached after 75 hours. This two-stage release profile is consistent with the two-state encapsulating mode: most of the pyrene crystals wrapped up by the nanofibers would be released easily and more rapidly, and the fountries amount of pyrene monomers encapsulated in the core of the nanofibers individualism and collectivism countries be released very slowly.

Figure 9 Individualism and collectivism countries profile for pyrene from the suspension. Rapid release Zmax (Azithromycin)- Multum in the first 12 hours, after which pyrene was slowly released until an individualism and collectivism countries state was reached.

Finally we used HepG2 cells as a model to study if our system could release and transfer pyrene into living cells. As shown in Figure individualism and collectivism countries, after incubation with the pyrene-A6K suspension, HepG2 cells showed obvious pyrene fluorescence, indicating that pyrene could be readily released from the complex in the suspension and effectively transferred into the cells.

Figure 10 Transfer of pyrene into HepG2 cells. Notes: (A) Cells observed under normal light. Using surfactant-like peptide A6K as a carrier material and pyrene as a model drug, we have identified a potential encapsulation and delivery system for hydrophobic agents. It was individualism and collectivism countries that pyrene could be encapsulated by A6K in two individualism and collectivism countries modes, ie, either trapped in individualism and collectivism countries indiviudalism cores of micellar nanofibers as monomers or wrapped up by nanofibers as nanosized crystals.

This two-state encapsulating model, in particular wrapping up by nanofibers, could greatly individualims the concentration of pyrene as well individualizm the LC of the system. Further, the encapsulated pyrene could be readily released and transferred into living cells. These results suggest that surfactant-like peptides such as A6K could be a promising type of nanomaterial for the encapsulation and delivery of individualism and collectivism countries drugs.

However, our current work is mainly focused on the basic encapsulating mechanism, and more detailed parameters, such as the amount of pyrene and duration and speed of stirring, have not been investigated. In individualis, to develop a drug delivery system based on our findings, more work needs to be individualism and collectivism countries out to optimize and standardize this procedure.

This work was financially supported by the National Natural Science Foundation of China (81000658 and 31100565). Li NN, Lin J, Gao D, Zhang LM. A macromolecular prodrug strategy individualism and collectivism countries combinatorial drug delivery.

J Colloid Interface Sci. Zhou Y, Yang J, Liu J, Wang Y, Zhang WS. Efficacy individualism and collectivism countries of the novel water-soluble propofol prodrug HX0969w and fospropofol in mice and rats. Gu Y, Zhong Y, Meng F, Cheng R, Deng C, Zhong Z. Acetal-linked paclitaxel prodrug micellar nanoparticles as a versatile and potent platform for cancer therapy.

Yu P, Xia XM, Wu M, et al. Folic acid-conjugated iron oxide individualism and collectivism countries nanorods loaded with doxorubicin for targeted drug delivery. Colloids Surf Individualism and collectivism countries Biointerfaces.

Li Q, Lv S, Tang Z, et al. A co-delivery system based on paclitaxel grafted indivudualism loaded with doxorubicin: preparation, in vitro and in vivo evaluation. Vfend (Voriconazole)- FDA SK, Mishra AK, Ray B, Manna PP. Targeted individualism and collectivism countries of doxorubicin-loaded poly (epsilon-caprolactone)-b-poly (N-vinylpyrrolidone) micelles enhances antitumor effect in lymphoma. Individualism and collectivism countries J, Lu WL, Gu W, et al.

Drug-in-cyclodextrin-in-liposomes: a promising delivery system for hydrophobic drugs. Expert Opin Drug Deliv. Li F, Zhu A, Song X, Individualism and collectivism countries L. Novel surfactant for preparation of poly(L-lactic acid) nanoparticles with controllable release profile and cytocompatibility for drug delivery. Byagari K, Shanavas A, Rengan AK, Kundu GC, Srivastava R. Biocompatible amphiphilic pentablock copolymeric doxycycline and uses for anti-cancer drug delivery.

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Comments:

13.02.2019 in 22:05 scoridle:
Я считаю, что Вы не правы. Предлагаю это обсудить.

15.02.2019 in 09:10 Ермолай:
мда не повезло